Publications

2022
Salah I, Adjroud O, Elwej A. Protective Effects of Selenium and Zinc Against Nickel Chloride-Induced Hormonal Changes and Oxidative Damage in Thyroid of Pregnant Rats. Biological Trace Element Research [Internet]. 2022;200 (5) :2183-2194. Publisher's VersionAbstract

Nickel chloride (NiCl2) is a heavy metal that may affect the function of the thyroid. Selenium (Se) and zinc (Zn) are essential trace elements involved in thyroid hormone metabolism. However, little is reported about thyrotoxicity during gestation. The current study aimed to investigate the protective effects of selenium and zinc against NiCl2-induced thyrotoxicity in pregnant Wistar rats. Female rats were treated subcutaneously (s.c.) on the 3rd day of pregnancy, with NaCl 0.9% and served as control, NiCl2 (100 mg/kg body weight (BW)) alone, or in association with Se (0.3 mg/kg, s.c.), ZnCl2 (20 mg/kg, s.c.), or both of them simultaneously. Oxidative stress parameters, thyroid biomarkers, and histopathological examination were evaluated. Results showed that NiCl2 exposure caused a significant decrease in maternal body weight and an increase in absolute and relative thyroid weight compared to the controls. NiCl2 administration also led to decreased plasma triiodothyronine (T3) and thyroxine (T4) with a concomitant significant increase in thyroid-stimulating hormone (TSH) levels when compared to that of control. In addition, an overall pro-oxidant effect was associated with a decrease in the reduced glutathione (GSH) and nonprotein thiol (NPSH) contents and the enzymatic activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD), and an increase in malondialdehyde (MDA). These biochemical disturbances were confirmed by histological changes. However, the co-treatment of Se and/or ZnCl2 attenuates NiCl2-induced changes. Our findings suggested that Se and ZnCl2 ameliorated NiCl2-induced thyrotoxicity in pregnant Wistar rats by exhibiting antioxidant effects.

Fedala A, Adjroud O, Bennoune O, Abid-Essefi S, Foughalia A, Timoumi R. Nephroprotective Efficacy of Selenium and Zinc Against Potassium Dichromate-Induced Renal Toxicity in Pregnant Wistar Albino Rats. Biological Trace Element Research [Internet]. 2022;200 (11) :4782-4794. Publisher's VersionAbstract

Hexavalent chromium (CrVI) compounds are potent toxicants commonly used in numerous industries. Thus, potential toxic effects and health hazards are of high relevance. Selenium (Se) and zinc (Zn) are known for their antioxidant and chemoprotective properties. However, little is known about their protective effects against CrVI-induced renal damage during pregnancy. In this context, the present study aimed to investigate the protective efficacy of these two essential elements against potassium dichromate-induced nephrotoxicity in pregnant Wistar Albino rats. Female rats were divided into control and four treated groups of six each receiving subcutaneously on the 3rd day of pregnancy, K2Cr2O7 (10 mg/kg, s.c. single dose) alone, or in association with Se (0.3 mg/kg, s.c. single dose), ZnCl2 (20 mg/kg, s.c. single dose) or both of them simultaneously. The nephrotoxic effects were monitored by the evaluation of plasma renal parameters, oxidative stress biomarkers, DNA damage, and renal Cr content. The obtained results showed that K2Cr2O7 disturbed renal biochemical markers, induced oxidative stress and DNA fragmentation in kidney tissues, and altered renal histoarchitecture. The co-administration of Se and/or ZnCl2 has exhibited pronounced chelative, antioxidant, and genoprotective effects against K2Cr2O7-induced renal damage and attenuated partially the histopathological alterations. These results suggest that Se and Zn can be used as efficient nephroprotective agents against K2Cr2O7-induced toxicity in pregnant Wistar Albino rats.

2021
Fedala A, Adjroud O, Abid-Essefi S, Timoumi R. The Mitigative Effect Of Selenium Against Hexavalent Chromium Induced- Toxicity In Liver Of Pregnant Wistar Rats. 1st International Seminar on Pollution, Health, Environment and Bio-monitoring SIPSEB’21 DECEMBER 27-28,. 2021.
Fedala A, Adjroud O. L’évaluation de la perturbation thyroïdienne induite par le dichromate de potassium chez la rate gestante ALBINOS WISTAR. 1er Séminaire International PERTURBATEURS ENDOCRINIENS ENVIRONNEMENT & REPRODUCTION (PEER 2021) tenu du 07 au 08 juillet. 2021.
Sengouga D, Adjroud O, Salah I, Fedala A. CHROMIUM TOXICITY ON THE ELECTROLYTE BALANCE AND ADRENAL HISTOLOGY IN WISTAR MALE RATS.  Nutr.Santé. 2021;10 (1).
Mammeri S, Chafai N, Harkat H, Rachida K, Chafaa S. Protection of Steel Against Corrosion in Acid Medium Using Dihydropyrimidinone Derivatives: Experimental and DFT Study. Iranian Journal of Science and Technology, Transactions A: Science [Internet]. 2021;45. Publisher's VersionAbstract

Two dihydropyrimidinone derivatives (DHPM) were synthesized via the multi-component reaction, the yield of the synthesis reached 90%, and their structures were proved by IR, 1H and 13C NMR spectroscopy. The identified molecules were used for studying the corrosion inhibition of XC48 carbon steel in 0.5 mol L−1 H2SO4 solution. The inhibitory potentials of these compounds were determined by electrochemical methods exploiting the potentiodynamic polarization curves (Tafel method) and electrochemical impedance spectroscopy. Surface phenomena have been illustrated by atomic force microscopy. The results obtained show that the synthesized molecules are effective inhibitors, whose efficiency corrosion inhibition depends on the architecture of their structure, and it is proportional to the concentrations; it exceeds 94% for one of these inhibitors. The adsorption of these compounds on the metal surface approached the Langmuir’s adsorption isotherm. Furthermore, the electronic properties obtained using density function theory are in a good consensus with the experimental efficiencies of inhibition. The molecular dynamics simulations have allowed also simulating the interactions between the inhibitors and the metallic surface.

Bouzghaia B, Ben Moussa M, Goudjil R, Harkat H, Pale P. Chemical composition, in vitro antioxidant and antibacterial activities of Centaurea resupinata subsp. dufourii (dostál) greuter. Natural Product Research [Internet]. 2021;35 (22) :1-5. Publisher's VersionAbstract

The current study focuses on the chemical composition, and evaluation of antioxidant and antibacterial activity of the aerial parts of Centaurea resupinata subsp. dufourii. Using different chromatographic methods nine compounds 1–9 were isolated. The structural identification of isolated compounds was achieved using several spectroscopic methods NMR techniques (1H NMR, 13C NMR, COSY, HSQC, HMBC) and mass spectroscopy (ESI-MS) and by comparison with literature data. The structures of these compounds were identified as nicotiflorin (1), apigetrin (2), chrysoeriol (3), apigenin (4), chrysin (5), daucosterol (6), β-sitosterol (7), taraxastrerol (8) and lupeol (9). The antibacterial and antioxidant activities of ethyl acetate and n-butanol extracts have been evaluated. The antioxidant activity was assessed in vitro using DPPH radical scavenging method, which showed that ethyl acetate extract possessed an interesting antioxidant potential (IC50 = 36.263 ± 0.005 μg/mL).

 

Graphical abstract

Fedala A, OUNASSA ADJROUD, Saouli A, Imane S. ZINC ALLEVIATES POTASSIUM DICHROMATE-INDUCED HEPATOTOXICITY IN PREGNANT WISTAR RATS. Malaysian Journal Of Biochemistry & Molecular Biology . 2021;2 :10-16 .Abstract

The present study aimed to investigate the potential protective effects of zinc (Zn) against hexavalent chromium-induced hepatotoxicity in pregnant Wistar rats. Female rats were treated subcutaneously (s.c) on the 3 rd day of pregnancy, with NaCl 0.9 % and served as control, K₂Cr₂O7 (10 mg/kg bw) alone, or K₂Cr₂O7 in association with ZnCl2 (20 mg/kg bw). Hepatic biochemical parameters, oxidative stress biomarkers and DNA damage were monitored. Results revealed that K₂Cr₂O7 disturbed plasma ALT, AST, ALP and GGT, induced hepatic oxidative stress and DNA fragmentation. The co-treatment with Zn has alleviated K2Cr2O7-induced hepatotoxicity by exhibiting antioxidant and genoprotective effects in pregnant Wistar rats.

Fedala A, Adjroud O, Abid-Essefi S, Timoumi R. Protective effects of selenium and zinc against potassium dichromate–induced thyroid disruption, oxidative stress, and DNA damage in pregnant Wistar rats. Environmental Science and Pollution Research. 2021;28 :22563–22576.Abstract

Hexavalent chromium (CrVI) is an environmental pollutant and an endocrine-disrupting metal. Se and Zn are essential trace elements, known to play a crucial role in thyroid homeostasis. However, there is a lack of data reporting thyrotoxicity during gestation. In this study, we investigated the protective effects of selenium and zinc against potassium dichromate–induced thyrotoxicity in pregnant Wistar rats. Thirty pregnant Wistar rats were divided into control and four treated groups receiving subcutaneously (s.c) on the 3rd day of pregnancy, K2Cr2O7 (10 mg/kg, s.c) alone, or in association with Se (0.3 mg/kg, s.c), ZnCl2 (20 mg/kg, s.c), or both of them simultaneously. The hormonal profile, oxidative stress biomarkers, DNA damage, and histological modifications were evaluated. Our main findings showed that K2Cr2O7 promoted hypothyroidism, oxidative stress, genotoxicity, and histological alterations in the thyroid gland. The co-treatment with Se or ZnCl2 has mitigated K2Cr2O7-induced thyrotoxicity in pregnant Wistar rats by exhibiting antioxidant and genoprotective effects. However, the combined co-treatment of both of them was less thyroprotective, and therefore, further investigations on the synergetic interaction of Se and Zn against CrVI toxicity using different doses and exposure routes are required.

Salah I, Adjroud O, Elwej A. Protective Effects of Selenium and Zinc Against Nickel Chloride–Induced Hormonal Changes and Oxidative Damage in Thyroid of Pregnant Rats. Biological Trace Element Research . 2021;155.Abstract

Nickel chloride (NiCl2) is a heavy metal that may affect the function of the thyroid. Selenium (Se) and zinc (Zn) are essential trace elements involved in thyroid hormone metabolism. However, little is reported about thyrotoxicity during gestation. The current study aimed to investigate the protective effects of selenium and zinc against NiCl2-induced thyrotoxicity in pregnant Wistar rats. Female rats were treated subcutaneously (s.c.) on the 3rd day of pregnancy, with NaCl 0.9% and served as control, NiCl2 (100 mg/kg body weight (BW)) alone, or in association with Se (0.3 mg/kg, s.c.), ZnCl2 (20 mg/kg, s.c.), or both of them simultaneously. Oxidative stress parameters, thyroid biomarkers, and histopathological examination were evaluated. Results showed that NiCl2 exposure caused a significant decrease in maternal body weight and an increase in absolute and relative thyroid weight compared to the controls. NiCl2 administration also led to decreased plasma triiodothyronine (T3) and thyroxine (T4) with a concomitant significant increase in thyroid-stimulating hormone (TSH) levels when compared to that of control. In addition, an overall pro-oxidant effect was associated with a decrease in the reduced glutathione (GSH) and nonprotein thiol (NPSH) contents and the enzymatic activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD), and an increase in malondialdehyde (MDA). These biochemical disturbances were confirmed by histological changes. However, the co-treatment of Se and/or ZnCl2 attenuates NiCl2-induced changes. Our findings suggested that Se and ZnCl2 ameliorated NiCl2-induced thyrotoxicity in pregnant Wistar rats by exhibiting antioxidant effects.

2020
Sengouga D, Adjroud O, Fedala A, Salah I. The antagonistic effect of the association of zinc and selenium on chromium induced toxicity in rat’s Testis. 1ere Journée Scientifique sur la Biologie des plantes Médicinales 22 Janvier. 2020.
Sengouga D, Adjroud O, Fedala A, Salah I. Hexavalent chromium - induced nephrotoxicity and its zinc and selenium antagonism in Wistar rat. Association nationale eco-conception, analyse de vie et développement durable affilié au réseau des clubs UNESCO en Algérie. Février. 2020.
Salah I, Adjroud O, Fedala A, Saouli A. Potential Protective Effects of Selenium and Zinc against Thyroid Toxicity Induced by Nickel in Preimplanted Wistar Albino Rats. Toxicology International [Internet]. 2020;26 (1) :54–63. Publisher's VersionAbstract

Nickel Is A Heavy Metal That May Affect The Function Of The Thyroid. The Present Study Was Undertaken To Assess The Protective Effects Of Selenium And Zinc Against Nickel-induced Thyrotoxicity In Preimplanted Wistar Albino Rats. Nickel Is Administered Alone (100 Mg/kg Body Weight) Subcutaneously Or In Combination With Selenium (0.3 Mg/kg Body Weight) Either With Zinc (20 Mg/kg Body Weight) Or Combined With Both Selenium And Zinc. All Groups Of Rats Were Injected On Day 3 Of Gestation In The Pre-implantation Period. Exposure Of Rats To Nickel Caused A Significant Decrease In Maternal Body Weight On Days 6 And 20 Of Gestation And An Increase In Absolute And Relative Thyroid Weight Compared To The Controls. Nickel Treatment Was Also Led In Decreased Plasma Triiodothyronine, Thyroxine With A Concomitant Significant Increase In Thyroid-stimulating Hormone Levels When Compared To Control. These Changes Were Confirmed By Histological Damages. However, Co-administration Of Selenium And/or Zinc To Nickel-treated Rats Restored The Hormonal And Histological Aspects. Our Findings Suggested That Selenium And Zinc Ameliorated Nickel Induced Thyroid Damage.

BOULILA I, Adjroud O. PROTECTIVE EFFECTS OF SELENIUM AND ZINC ON NICKEL CHLORIDE INDUCED REPRODUCTIVE TOXICITY IN WISTAR ALBINOS PREIMPLANTED RATS. Studia Universitatis Vasile Goldis Seria Stiintele Vietii (Life Sciences ) . 2020;30 (3) :126 - 135.Abstract

: The aim of this study was to investigate that nickel chloride (NiCl2) induced reproductive toxicity in pre- implanted Wistar Rats and examined the possible protective effect of zinc chloride and selenium on plasma concentration of the hormones of 17 b etradiol (E2) and progesterone (prog); on the reproductive organ’s histology and on development. Experimental results showed the subcutaneous (s.c) administration of Nicl2 to Wistar albino Rats induced a decrease in plasma concentration of E2 and prog in addition, disturbance in development parameters and structural damages to the histology of the reproductive organs. Conversely, Se and ZnCl2 dues to the antioxidants property, regulate the secretion of E2 and Prog hormones, prevent alterations in the reproductive organs and in development in preimplanted rates receiving NiCl2.

2019
Sengouga D, Adjroud O, Salah I, Fedala A. Protective effect of zinc and selenium against chromium hematotoxicity and water consumption in the rats. 1ere Journée de Biotoicologie et Bioactivité, 27 Nov. 2019.
ANFAL FEDALA, Adjroud O, Saouli A. Evaluation de l’hepatotoxicite induite par le dichromate de potassium chez les rates gestantes de la souche albinos wistar. 2019.
Sengouga D, Adjroud O, Salah I, Fedala A. Protective effect of zinc and selenium against chromium hematotoxicity and water consumption in the rats. 1ere Journée de Biotoicologie et Bioactivité, 27 nov,. 2019.
Ouache R, Harkat H, Pale P, Oulmi K. Phytochemical compounds and anti-corrosion activity of Veronica rosea. Natural Product Research [Internet]. 2019;33 :1-5. Publisher's VersionAbstract

The aim of this work is the phytochemical study of the butanolic extract of the aerial parts of Veronica rosea. Four compounds 14 have been isolated using different chromatographic methods. The structures of these compounds were determined by NMR spectral analysis and mass spectroscopy. The adsorption and anticorrosion effects of this extract were investigated towards the corrosion of copper in 1 M HNO3 aqueous by the weight loss technique and potentiodynamic polarization. The results showed that the butanolic extract is a good inhibitor and the inhibition efficiency increases with increasing of concentration of the inhibitor. The adsorption of this extract on the copper specimen surface was spontaneous and obeyed the Langmuir’s adsorption isotherm. Large value of adsorption equilibrium Constant (K ads = 35 L g−1) was obtained. The polarization experiments confirmed the data obtained by gravimetric weight-loss. Tafel plot of polarization curves indicates that the extract acts as a mixed type inhibitor.

Graphical abstract

Kala K, Soltani F, Saidi M. Les complications chroniques chez les patients drépanocytaires. Congrès maghrébin d'Hématologie , Octobre . 2019.
Bouabdallah N, Kacha F, Saidi M. Lymphomes T cutanés primitifs : à propos de 4 cas. . Congrès maghrébin d'Hématologie , Octobre . 2019.

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