Human rickettsioses in the Batna area, eastern Algeria

Citation:

Mokrani, Khamsa, et al. 2012. “Human rickettsioses in the Batna area, eastern Algeria”. Jounal of ScienceDirect, Ticks and Tick-borne Diseases 152, ISSN: 1877-9603, PMID: 23182273, Dec 3 (5-6) : pp 364-366.

Abstract:

In order to investigate the prevalence of rickettsioses in febrile exanthemas in eastern Algeria, we conducted a prospective serological analysis of all patients presenting with this clinical picture at the Infectious Diseases Department in the Batna Hospital from January 2000 to September 2006. One hundred and eight adult patients were included in the study, 46% of whom younger than 25 years, and 72.5% were admitted from May to September. Patients were tested for antibodies to Rickettsia conorii conorii, R. conorii israelensis, R. aeschlimannii, R. felis, R. sibirica mongolitimonae, R. africae, R. massiliae, R. typhi, and R. prowazekii using microimmunofluorescence and Western blot as confirmation procedure. Both methods confirmed the diagnosis ofMediterranean spotted fever in 5 patients (4.6%), flea spotted fever in 2 patients (1.9%), and R. aeschlimannii infection in 2 patients (1.9%). In addition, 4 patients were diagnosed as having murine typhus (3.7%) and one with epidemic typhus (0.9%). To the best of our knowledge, this is the first report ofR. felis infection and R. aeschlimannii infection in Algeria. In conclusion, at least 5 different kinds of rickettsiosis coexist in eastern Algeria and, being responsible for 13% of cases of febrile exanthemas, should be considered among major causes of this clinical picture in this area. In addition, we emphasize the high incidence of typhus group rickettioses, includingepidemic typhus, in this area.

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Carcinome hépatocellulaire: expérience du centre de radiothérapie de Batna, ISSN/e- ISSN 2437-0665 / 2437-0665

Kamel, Hamizi, et al. 2018. “Carcinome hépatocellulaire: expérience du centre de radiothérapie de Batna, ISSN/e- ISSN 2437-0665 / 2437-0665”. ournal of medical sciences volume 5 : pp 74-77.

The predominance of codon 39 (c>t) mutation of HBB gene in a portion of the Algerian population (Northeast Algeria)

Kamilia, Belhadi, et al. 2017. “The predominance of codon 39 (c>t) mutation of HBB gene in a portion of the Algerian population (Northeast Algeria)”. Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale volume 90 (ssue 1) : pp 13-18. Publisher's Version Abstract

This study was planned to determine the frequency of β-thalassemia mutations in Batna region (Northeast Algeria). Nineteen blood samples of clinically thalassemic children patients were collected from Department of Pediatrics, University Hospital of Batna. We carried out the molecular genetics of beta globin gene by the method of minisequencing using Snapshot™ kit (Applied Biosystems) in search of the four most common HBB genetic variants including three β-thalassemia mutations: codon 39(C>T) (HBB: c.118C>T), IVSI-110(G>A) (HBB: c.93-21G>A), and IVSI-1-2(T>G) (HBB: c.92+2T>G), as well as the hemoglobin S variant (HBB: c.20A>T). We used direct DNA sequencing to detect the rare mutations of beta-globin gene. We have revealed the presence of four different β-globin gene mutations responsible for β-thalassemia in Batna region. According to our results, the nonsense mutation at codon 39 (C>T) is the most frequent mutation type in our province, the same as other geographical regions of Algeria. It is followed by codon 54(-T), detected in a second Algerian family (the proband was homozygote), and the first association of Hb Knossos: codon 27 (G>T) allele with codon 39 (C>T) in the Algerian population. Here we reportws also the association of codon 39(C>T) with IVS-I-110 (G>A). Our preliminary results show the predominance of codon 39 (c>t) mutation of HBB gene in Batna region

Effects of Codon 39 (c>t) Mutation on The Changes of Hematological Parameters in Children with Beta-Thalassemia Major in the Region of Batna, Algeria. ISSN 0971-9032

Belhadi, Kamilia, et al. 2016. “Effects of Codon 39 (c>t) Mutation on The Changes of Hematological Parameters in Children with Beta-Thalassemia Major in the Region of Batna, Algeria. ISSN 0971-9032”. Current Pediatric Research volume 20 (Issue 1) : pp 203-208. Publisher's Version Abstract

Clinical heterogeneity is often due to genetic heterogeneity. One mechanism by which different mutations can produce a phenotypic variation). Beta-thalassemia is mainly caused by a large number of mutations of the gene for beta-globin. The objective of this study is to correlate molecular lesions and clinical signs in children with beta-thalassemia homozygous. We carried out the molecular genetics of beta globin gene by the method of mini sequencing using Snapshot ™ kit (Applied Biosystems) in search of the mutation codon 39 (C>T) (HBB: c.118C>T) and we studied haematological parameters by statistical method using the test “t” of Student for comparing the means of the samples. The peripheral blood smear examination was done from the slides stained in Wright stains. The results obtained have shown that beta-thalassemia homozygous children with severe anemia over 30 ± 0.9 g/L of total Hb with microcytosis of 60,80 ± 2.014 fl, hypochromia 18.47 ± 0.6023 pg and the number of red blood cells is 3.220 ± 0.2778 (10¹²/L). In addition, a significantly secondary thrombocytosis and leukocytosis were reported in patients. Thus the electrophoresis of hemoglobin exhibits a high Hb F (55 to 82%). The peripheral smear examination revealed many red cell abnormalities in the blood. In this study, we used the mini sequencing assay as a rapid screening procedure to identify the severe codon 39 (C>T) mutation in the HBB gene. Phenotype of beta thalassemia major is characterized with various hematological parameters.

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Thrombose révélatrice d'hémopathie maligne

Yamina, Ouarlent, and H Liadhi. 2014. “Thrombose révélatrice d'hémopathie maligne”. Journal of medical sciences vol 3 (n°2) : pp 132-133.

Laboratoire des Hépatites Virales

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