Citation:
Abstract:
Biologically active compounds isolated from medicinal herbs have been the center of interest for researchers to investigate their possible effects and mechanisms through which they exert their action. In the current study, we investigated the antiproliferative effect and the mechanism of action of deoxypodophyllotoxin, a semi-synthetic compound derived from the extract of a Chinese herbal medicine, Dysosma versipellis (Hance) M. Cheng ex Ying (Berberidaceae). The study was conducted on MCF-7 and MDA-MB-231 breast cancer cell lines. The antiproliferative effect of deoxypodophyllotoxin was assessed by the Cell Counting Kit-8 assay. Flow cytometry, Annexin V/PI, mitochondrial membrane potential, caspase inhibition assays, and western blot analysis were performed to detect, explore, and assess the antiproliferative effect of deoxypodophyllotoxin. Our data revealed that, deoxypodophylloto…